Tiger 3, released in 2023, starring Salman Khan, and directed by Mankeesh Sharma, is the fifth movie in the YRF Spy Universe. It follows Tiger in a battle against an old foe with a personal vendetta.
Spoilers below.

Short answer
No, the horses are fine and never harmed.
Detailed answer
An explosive is thrown near horses in a stable. While this panics the horses and causes them to run out of their stables, none of them are harmed. Some gunfire takes place as the horses run, but again, no horses are harmed during this sequence.
What is Tiger 3
Tiger 3 is 2023 Hindi action-espionage film. It stars Salman Khan (reprising the titular role), Katrina Kaif, and Emraan Hashmi. The story follows a series of events that take place when an old enemy of Tiger's returns to settle a personal grudge.
If you're interested in my thoughts of the film, please see the Tiger 3 movie review.
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